GFP polyclonal antibody
产品名称： GFP polyclonal antibody
英文名称： GFP polyclonal antibody
产品编号： PAB1264
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against a native GFP.
- Immunogen:
- Native purified EGFP.
- Host:
- Rabbit
- Specificity:
- This antibody recognizes GFP, EGFP, EYFP fusion proteins in all species.
- Form:
- Liquid
- Concentration:
- 1 mg/mL
- Storage Buffer:
- In PBS, pH 7.4 (15 mM sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
- Immunofluorescence
- Confocal microscopy images of COS - 7 cells transfected with expression constructs encoding membrane - tethered EGFP (membrane - EGFP ; top) or nuclear Polycomb 2 - EYFP fusion protein (Pc2 - EYFP ; bottom). The natural fluorescence of the produced proteins is shown in the green channel (left), GFP polyclonal antibody (Cat # PAB1264) signal was detected in the red channel (right). The system was carefully tested for overlap of these two optical channels and images were scanned separately in sequential scanning mode. The blue nuclear stain is also shown.
- Immunoprecipitation
- Immunoprecipitation of GFP-NLS from HEK293 cells using GFP polyclonal antibody (Cat # PAB1264). HEK293 cells were transfected with expression construct encoding GFP-NLS protein. Twenty hours post transfection cells were lysed in non-denaturating conditions (Lysis buffer: 20 mM Tris, pH 7.5, 100 mM NaCl, 0.5% Triton X-100, inhibitors of proteases). Aliquots of cell lysate were immunoprecipitated using a polyclonal GFP polyclonal antibody (Lane 2) or a pre-immune rabbit serum (Lane 3). Immunoprecipitates together with a sample of the cell lysate (Lane 1) were separated on SDS-PAGE polyacrylamide gel and immunoblotted with the GFP polyclonal antibody. The positions of molecular weight markers in kDa are indicated at the left.